环境微量污染物与健康效应的生物传感原理与关键技术，包括生物识别材料设计与筛选、光学生物传感器、效应导向生物传感分析技术、CRISPR生物分析化学等

1. 国家自然科学基金重大项目，环境介质中的病毒识别与传播规律，2021/01-2025/12，在研，参加

2. 清华大学春风基金抗疫专项，CRISPR/Cas13a结合核酸自驱动放大的病毒现场检测试纸条及其定量检测装置，2020/09-2022/09，在研，主持

3. 中国工程院应急科技攻关项目，城市水系统关键节点新型冠状病毒存在风险识别，2020/02-2021/02，结题，参加

4. 国家自然科学基金面上基金项目，分子对接辅助优化水中环境雌激素拟/抗活性同步检测的生物传感分析技术，2020/01-2023/12，在研，主持

5. 国家重点研发计划，村镇社区环境监测及修复关键技术研发，2019/11-2022/12，在研，参加

6. 国家重点研发计划，场地地下水污染快速识别与风险监测管控技术，2020/01-2022/12，在研，参加

7. EPSC自由申请课题，微囊藻毒素广谱性抗体筛选及其倏逝波免疫传感分析应用，2019/08-2021/08，结题，主持

8. 北京市自然科学基金-海淀原始创新联合基金重点项目，水中抗生素快速定量检测的倏逝波生物传感分析技术，2019/01-2021/12，结题，主持

9. 清华大学-剑桥大学联合科研基金，功能化二维材料表面等离子体共振增强机制及其用于环境雌激素传感分析研究，2019/01-2021/07，结题，主持

10. 北京市自然科学基金面上项目，基于受体的再生水中雌激素效应检测倏逝波荧光传感技术，2018/01-2019/12，结题，主持

11. EPSC联合基金，核受体介导的环境内分泌干扰物效应检测生物传感器技术，2017/05-2019/04，结题，联合主持

12. 国家自然科学基金中英合作交流项目，用于环境生物检测的多通道集成光波导芯片，2017/04-2019/03，结题，主持

13. 国家自然科学基金面上基金项目，基于工程化适配体的水中抗生素快速定量倏逝波传感检测技术，2017/01-2020/12，结题，主持

14. 国家科技部重大仪器开发专项，多指标平面波导生物芯片的开发，2012/09-2017/09，结题，参与

15. 国家科技部863计划，水质安全生物预警监测技术与系统，2014/01-2017/12，结题，参加

16. 北京市自然科学基金项目，基于微电极测试的污水深度处理反硝化生物滤池脱氮模型，2013/01-2015/12，结题，主持

17. 清华大学国际科技合作项目，基于功能核酸的重金属检测技术研究，2013/01-2014/12，结题，主持

18. 国家自然科学基金青年基金，人工金属氧化物纳米颗粒在污水生物处理系统中的归趋及对微生物聚集体活性的影响，2012/01-2014/12，结题，负责人

2021年 总微囊藻毒素在线分析仪，中国发明协会发明创业奖创新奖二等奖/第48届日内瓦国际发明展会金奖，排名第一

2020年 中国产学研合作创新奖、中国仪器仪表学会朱良漪分析仪器青年创新奖、国家生态环境保护专业技术青年拔尖人才

2015年 清华大学环境学院学生工作先进个人

2014年 微量有毒污染物快速高灵敏检测的生物传感技术及仪器，教育部技术发明奖一等奖，排名第六

2014年 入选“清华大学桑坦德银行资助项目”

2014年《污水处理在线监测仪器原理与应用（第二版）》获得“2014年中国石油和化学工业优秀出版物奖·图书奖”二等奖（排名第二）

2011年 教育部“长江学者和创新团队发展计划”创新团队，成员

以第一/通讯作者在Nature Communications、Light: Science & Applications、B&B、EST等发表SCI论文100余篇，论文H-index为37；以第一发明人授权国家发明专利9项，实现转化与应用3项。

近期发表论文（^#共同一作；^*通讯）：

1. Jin, YS, Sun, ZJ, Gu, AZ, Zhou, XH. Proteomics technologies in toxicity screening: a review[J]. ENVIRONMENTAL CHEMISTRY LETTERS, Published 2025, Volume 23, Issue 1, Pages 67-80

2. Jiao, SP, Jin, YS, Lichtfouse, E, Zhou, XH. Hydrovoltaic technologies for self-powered sensing and pollutant removal in water and wastewater: a review[J]. ENVIRONMENTAL CHEMISTRY LETTERS, Published 2025

3. Qian, XN, Zhu, QY, Sun, WJ, Zhou, XH, Xiong, XY, Zeng, SY, Sheng, JW, He, M. A reagent-free on-site COD monitoring by a variable optical path UV-Vis spectrometer[J]. FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING Published 2025, Volume 19, Issue 5

4. Wang, YD, Chen, J, Gong, LK, Tang, JJ, Wang, XW, Guo, HY, Zhou, XH. P-doped W2C nanoparticles for hydrogen evolution reaction powered by a wind-driven triboelectric nanogenerator[J]. NANO ENERGY, Published 2024, Volume 121

5. Yang, YH, Sun, LL, Zhao, JH, Jiao, Y, Han, TL, Zhou, XH. Improving trans-cleavage activity of CRISPR-Cas13a using engineered crRNA with a uridinylate-rich 5′-overhang[J]. BIOSENSORS & BIOELECTRONICS, Published 2024, Volume 255

6. Wang, Bohan,Liu, Lanhua,Zhang, Haopeng,Wang, Zhiqiang,Chen, Kang,Wu, Bo,Hu, Limin,Zhou, Xiaohong,Liu, Lanlan. A group-targeting biosensor for sensitive and rapid detection of quinolones in water samples[J]. Analytica Chimica Acta, Published 2024, Volume 1301

7. Yang, YH, Tan, JS, Wang, F, Sun, WM, Shi, HC, Cheng, Z, Xie, YC, Zhou, XH. Preconcentration and detection of SARS-CoV-2 in wastewater: A comprehensive review[J]. BIOSENSORS & BIOELECTRONICS, Published 2024, Volume 263

8. Zhou, Xiaohong,Zhu, Qian,Zheng, Anqi,Xue, Boyuan,Wang, Qihui,Chin, Lip Ket,Jiang, Jingkun,He, Miao. De novo design of covalent bonding peptides for target protein [J]. hLife, Published 2024, Volume 2, Issue 12, Pages 641-652

9. Liu, JC, Yang, H, Li, HX, Wang, JC, Zhou, XH. A colorimetric nanobiosensor with enhanced sensitivity for detection of lead (II) in real-water samples via an adenine-cytosine mismatched DNAzyme[J]. FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING, Published 2024, Volume 18, Issue 12

10. Xue, BY, Li, RX, Cheng, Z, Zhou, XH. High-Affinity Peptides for Target Protein Screened in Ultralarge Virtual Libraries[J]. ACS CENTRAL SCIENCE, Published 2024, Volume 10, Issue 11, Pages 2111-2118

11. Jin, YS, Xue, BY, Zhou, XH. Protein Biomarkers of DNA Damage in Yeast Cells for Genotoxicity Screening[J]. ENVIRONMENT & HEALTH, Published 2024, Volume 3, Issue 3, Pages 250-258

12. Liu, JC, Xing, YP, Lin, YS, Xie, YC, Zhou, XH. Effect of pretreatment approach on the ELISA-based detection of cyanotoxins in water: Analysis and application[J]. SCIENCE OF THE TOTAL ENVIRONMENT, Published 2023, Volume 871

13. Tan, Jisui, Chen, Yangyang, He, Jing, Occhipinti, Luigi G., Wang, Zhanhui, Zhou, Xiaohong. Two-dimensional material-enhanced surface plasmon resonance for antibiotic sensing[J]. Journal of Hazardous Materials[0304-3894], Published 2023, Volume 455

14. Wang, Xingwei, Zhou, Wenbo, Wang, Yidi, Gong, Likun, Liu, Xiaobin, Zhou, Xiaohong. MoO2/Mo heterostructures for hydrogen evolution reaction and ammonia sensing in self-powered mode[J]. Nano Energy, Published 2023, Volume 109

15. Li, Fangxu, Yang, Yihan, Tan, Jisui, Wang, Zhanhui, Zhou, Xiaohong. Group-targeting sulfonamides via an evanescent-wave biosensor based on rational designed coating antigen[J]. Science of the Total Environment, Published 2023, Volume 861

16. Dai, Zongren, Tan, Jisui, Zhou, Kaiming, Zhang, Lin, Zhou, Xiaohong, Tan, Yidong. Optical fiber SPR biosensor with frequency multiplexing compensated laser heterodyne feedback for ultrasensitive detection of fluoroquinolones[J]. Sensors and Actuators B: Chemical, Published 2023, Volume 393

17. Yang, Yihan, Wang, Fan, Xue, Boyuan, Zhou, Xiaohong. Field-deployable assay based on CRISPR-Cas13a coupled with RT-RPA in one tube for the detection of SARS-CoV-2 in wastewater[J]. Journal of Hazardous Materials, Published 2023, Volume 459

18. Yang, Qian, Zhu, Qian, Li, Fangxu, Gui, Ping, Zhang, Ruina, Liu, Chun, Zhou, Xiaohong. Genotoxicity detection technology and application based on recombinant luminescent yeast 基于重组发光酵母的遗传毒性检测技术及应用[J]. Huanjing Kexue Xuebao/Acta Scientiae Circumstantiae, Published 2023, Volume 43, Issue 8, Pages 464-472

19. Lin, Yongshu, Liu, Jinchuan, Tian, Yongjing, Huang, Tianyin, Hu, Guanjiu, Zhou, Xiaohong. Comparison of pre-treatment methods for microcystins detection based on the immunoassay 总微囊藻毒素免疫检测及其预处理方法研究[J]. Huanjing Kexue Xuebao/Acta Scientiae Circumstantiae, Published 2023, Volume 43, Issue 8, Pages 437-479

20. Tan, Jisui, Dai, Zongren, Zhou, Kaiming, Zhang, Lin, Zhou, Xiaohong, Tan, Yidong. Ultrasensitive Screening of Endocrine-Disrupting Chemicals Using a Surface Plasmon Resonance Biosensor with Polarization-Compensated Laser Heterodyne Feedback[J]. Analytical Chemistry, Published 2023, Volume 95, Issue 22, Pages 8687-8695

21. Tan, Jisui, Dai, Zongren, Zhou, Kaiming, Zhang, Lin, He, Miao, Tan, Yidong, Zhou, Xiaohong. An Ultrasensitive and Universal Surface Plasmonic Biosensor for Detection of Micropollutants in Aquatic Environments[J]. Environmental Science and Technology, Published 2023, Volume 57, Issue 22, Pages 8313-8322

22. Tan, Jisui, Zhou, Xiaohong. Detection of Retinoic Acid-Active Chemicals in Diverse Sample Matrices Via a Quantum Dots-Based Nuclear Receptor Fluorescence Probe-Mediated Biosensor[J]. Analytical Chemistry, Published 2023, Volume 95, Issue 20, Pages 8036-8044

23. Xue, Boyuan, Yang, Qian, Xia, Kaidong, Li, Zhihong, Chen, George Y., Zhang, Dayi, Zhou, Xiaohong. An AuNPs/Mesoporous NiO/Nickel Foam Nanocomposite as a Miniaturized Electrode for Heavy Metal Detection in Groundwater[J]. Engineering[2095-8099], Published 2023, Volume 27, Pages 199-208

24. Tan, JS, Li, FX, Liu, LH, Zhang, J, Gui, P, He, M, Zhou, XH. Effect-Targeted Mapping of Potential Estrogenic Agonists and Antagonists in Wastewater via a Conformation-Specific Reporter-Mediated Biosensor[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, Published 2023, Volume 57, Issue 41, Pages 15617-15626

25. Xue, BY, Yang, Q, Jin, YS, Zhu, Q, Lan, JQ, Lin, YS, Tan, JS, Liu, LH, Zhang, T, Chirwa, EMN, Zhou, XH. Genotoxicity Assessment of Haloacetaldehyde Disinfection Byproducts via a Simplified Yeast-Based Toxicogenomics Assay[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, Published 2023, Volume 57, Issue 44, Pages 16823-16833

26. H.H. Zhu, J. Zou, H. Zhang, Y.Z. Shi, S.B. Luo, N. Wang, H. Cai, L.X. Wan, B. Wang, X.D. Jiang^*, J. Thompson, X.S. Luo, X.H. Zhou^*, L.M. Xiao^*, W. Huang, L Patrick, M. Gu^*, L.C. Kwek^*, and A. Q. Liu^*. Space-efficient optical computing and an integrated chip of diffractive neural network. Nature Communications, 2022, 13, 1044.

27. Qian Zhu, Xiaohong Zhou^*. A colorimetric sandwich-type bioassay for SARS-CoV-2 using a hACE2-based affinity peptide pair. Journal of Hazardous Materials, 2022, 425, 127923.

28. Jinchuan Liu, Yunpeng Xing, Boyuan Xue, Xiaohong Zhou^*. Nanozyme enhanced paper-based biochip with a smartphone readout system for rapid detection of cyanotoxins in water. Biosensors and Bioelectronics, 2022, 205, 114099.

29. Lanhua Liu^#, Xuejun Zhang^#, Qian Zhu^#, Kaiwei Li, Yun Lu, Xiaohong Zhou^*, Tuan Guo^*. Ultrasensitive detection of endocrine disruptors via superfine plasmonic spectral combs. Light: Science and Applications, 2021, 10, 181.

30. Qian Zhu, Lanhua Liu, Ruoyu Wang, Xiaohong Zhou^*. A split aptamer (SPA)-based sandwich-type biosensor for facile and rapid detection of streptomycin. Journal of Hazardous Materials, 2021, 403, 123941.

31. Yihan Yang, Jinchuan Liu, Xiaohong Zhou^*. A CRISPR-based and post-amplification coupled SARS-CoV-2 detection with a portable evanescent wave biosensor. Biosensors and Bioelectronics, 2021, 190, 113418.

32. Jinchuan Liu, Yihan Yang, Qian Zhu, Zhanhui Wang, Guanjiu Hu, Hanchang Shi, Xiaohong Zhou^*. ELISA-Based Method for Variant-Independent Detection of Total Microcystins and Nodularins via a Multi-immunogen Approach. Environmental Science & Technology, 2021, 55, 19, 12984-12993.

33. Jinchuan Liu, Yunpeng Xing, Xiaohong Zhou^*, George Y. Chen^*, Hanchang Shi. Light-sheet skew rays enhanced U-shaped fiber-optic fluorescent immunosensor for microcystin-LR. Biosensors and Bioelectronics, 2021, 176, 112902.

34. Yangyang Chen^#, Qian Zhu^#, Xiaohong Zhou^*, Ruoyu Wang, Zhenchuan Yang. Reusable, facile, and rapid aptasensor capable of online determination of trace mercury. Environmental International, 2021, 146, 106181.

35. Jinbiao Ma, Manman Du, Can Wang^*, Xinwu Xie^*, Hao Wang, Tie Li^*, Shixing Chen, Lixia Zhang, Shun Mao, Xiaohong Zhou^*, Min Wu. Rapid and sensitive detection of Mycobacterium tuberculosis by an enhanced nanobiosensor. ACS Sensors, 2021, 6(9), 3367-3376.

36. Xiaohong Zhou^*, Abdul Ghaffar Memon, Weiming Sun, Fang Fang, Jinsong Guo^*. Fluorescent probe for Ag+ detection using SYBR GREEN I and C-C mismatch. Biosensors, 2021, 11, 6.

37. Weiming Sun, Lanhua Liu, Abdul Ghaffar Memon, Xiaohong Zhou^*, Hongwei Zhao^*. Waveguide-based fluorescent immunosensor for the simultaneous detection of carbofuran and 3-hydroxy-carbofuran. Biosensors, 2020, 10, 191. Selected as Title Story.

38. Xiaohong Zhou^*, Qian Zhu, Yihan Yang. Aptamer-integrated nucleic acid circuits for biosensing: Classification, challenges and perspectives. Biosensors and Bioelectronics, 2020, 165, 112422.

39. Xuewen Yi, Zhanqi Gao, Lanhua Liu, Qian Zhu, Guanjiu Hu^*, Xiaohong Zhou^*. Acute toxicity assessment of drinking water source with luminescent bacteria: Impact of environmental conditions and a case study in Luoma Lake, East China. Frontier Environmental Science and Engineering, 2020, 14(6), 109.

40. Yunpeng Xing, Qian Zhu, Xiaohong Zhou^*, Peishi Qi. A dual-functional smartphone-based sensor for colorimetric and chemiluminescent detection: A case study for fluoride concentration mapping. Sensors and Actuators B: Chemical, 2020, 319, 128254.

41. Yunpeng Xing, Qian Zhu, Xiaohong Zhou^*, Peishi Qi^*. A gold nanoparticle-based colorimetric mercury(II) biosensor using a DNA probe with phosphorothioate RNA modification and exonuclease III-assisted signal amplification. Microchimica Acta, 2020, 187, 214.

42. Abdul Ghaffar Memon, Yunpeng Xing, Xiaohong Zhou^*, Ruoyu Wang, Lanhua Liu, Siyu Zeng^*, Miao He^*, Mei Ma. Ultrasensitive colorimetric aptasensor for Hg2+ detection using Exo-III assisted target recycling amplification and unmodified AuNPs as indicators. Journal of Hazardous Materials, 2020, 384, 120948.

43. Ruoyu Wang^#, Qiansen Zhang^#, Yi Zhang, Hanchang Shi, Kim Truc Nguyen, Xiaohong Zhou^*. Unconventional split aptamers cleaved at functionally essential sites preserve biorecognition capability. Analytical Chemistry, 2019, 91, 15811-15817. Selected as Supplementary Cover Article.

44. Kaidong Xia, Zhihong Li, Xiaohong Zhou^*. Ultrasensitive detection of a variety of analytical targets based on a functionalized low-resistance AuNPs/beta-Ni(OH)₂ nanosheets/Ni foam sensing platform. Advanced Functional Materials, 2019, 29, 1904922.

45. Ruoyu Wang, Xiyu Zhu, Yunpeng Xing, Abdul Ghaffar Memon, Hanchang Shi, Xiaohong Zhou^*. Multitag-regulated cascade reaction: A generalizable ultrasensitive microRNA biosensing approach for cancer prognosis. ACS Applied Materials & Interface, 2019, 11, 36444-36448.

46. Yangyang Chen, Jinchuan Liu, James S. Wilkinson^*, Xiaohong Zhou^*. Optical biosensors based on refractometric sensing schemes: A review. Biosensors and Bioelectronics, 2019, 144, 111693.

47. Ruoyu Wang, Xueqi Wu, Xiyu Zhu, Hanchang Shi, Xiaohong Zhou^*. A photoregulated split aptaswitch for small molecules with improved sensitivity. Chemical Communications, 2019, 55, 9555-9558.

48. Lanhua Liu, Xiaohong Zhou^*, Yun Lu, Hanchang Shi, Mei Ma, Tong Yu. Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples. Environment International, 2019, 132, 105091.

49. Qian Zhu, Lanhua Liu, Xiaohong Zhou^*, Mei Ma. In silico study of molecular mechanisms of action: Estrogenic disruptors among phthalate esters. Environmental Pollution, 2019, 255, 113293.

50. Xiyu Zhu, Ruoyu Wang, Kaidong Xia, Xiaohong Zhou^*, Hanchang Shi. Nucleic acid functionalized fiber optic probes for sensing in evanescent wave: optimization and application. RSC Advances, 2019, 9, 2316-2324.

51. Abdul Ghaffar Memon, Xiaohong Zhou^*, Yunpeng Xing, Ruoyu Wang, Lanhua Liu, Mohsin Khan, Miao He^*. Label-free colorimetric nanosensor with improved sensitivity for Pb²⁺ in water by using a truncated 8-17 DNAzyme. Frontiers of Environmental Science & Engineering, 2019, 13, 12.

52. Lanhua Liu^#, Didi Shan^#, Xiaohong Zhou, Hanchang Shi, Baodong Song, Floris Falke, Arne Leinse, R.G. Heideman. TriplexTM waveguide-based fluorescence biosensor for multichannel environmental contaminants detection. Biosensors and Bioelectronics, 2018, 106, 117-121.

53. Jinchuan Liu, Xiaohong Zhou^*, Hanchang Shi. An optical biosensor-based quantification of microcystin synthetase A gene: Early warning of toxic cyanobacterial blooming. Analytical Chemistry, 2018, 90, 2362-2368.

54. Lanhua Liu, Xiaohong Zhou^*, Ruiman Ma, Miao He, Hanchang Shi, Qian Yi. High-throughput biomolecular interaction analysis probing by an array fluorescent biosensor platform, Sensors and Actuators B: Chemical, 2018, 259,888-893.

55. Qian Zhu, Lanhua Liu, Yunpeng Xing, Xiaohong Zhou^*. Duplex functional G-quadruplex/NMM fluorescent probe for label-free detection of lead(II) and mercury(II) ions, Journal of Hazardous Materials, 2018, 355, 50-55.

56. Ruoyu Wang, Xiaohong Zhou^*, Bo Liedberg, Xiyu Zhu, Abdul Ghaffar Memon, Hanchang Shi. Screening criteria for qualified antibiotic targets in unmodified gold nanoparticles-based aptasensing. ACS Applied Materials & Interfaces, 2017, 9, 35492–35497.

57. Xiyu Zhu, Ruoyu Wang, Xiaohong Zhou^*, Hanchang Shi. Free-energy-driven lock/open assembly-based optical DNA sensor for cancer-related microRNA detection with a shortened time-to-result, ACS Applied Materials & Interface, 2017, 9, 25789-25795.

58. Lanhua Liu, Xiaohong Zhou*, Yun Lu^*, Didi Shan, Bi Xu, Miao He, Hanchang Shi, Yi Qian. Facile screening of potential xenoestrogens by an estrogen receptor-based reusable optical biosensor. Biosensors and Bioelectronics, 2017, 97, 16-20.

59. Lanhua Liu, Xiaohong Zhou^*, James S. Wilkinson, Ping Hua^*, Baodong Song, Hanchang Shi. Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: optimization and analysis. Scientific Reports, 2017, 7: 3655.

60. Ruoyu Wang, Xiaohong Zhou^*, Xiyu Zhu, Chao Yang, Lanhua Liu, Hanchang Shi. Isoelectric bovine serum albumin: Robust blocking agent for enhanced performance in optical-fiber based DNA sensing. ACS Sensors. 2017, 2(2): 257–262.

61. Abdul Ghaffar Memon, Xiaohong Zhou^*, Jinchuan Liu, Ruoyu Wang, Lanhua Liu, Bofan Yu, Miao He^*, Hanchang Shi. Utilization of unmodified gold nanoparticles for label-free detection of mercury (II): Insight into rational design of mercury-specific oligonucleotides. Journal of Hazardous Materials, 2017, 321, 417-423.

62. Lanhua Liu, Xiaohong Zhou^*, Mifang Lu, Mingkai Zhang, Chao Yang, Ruiman Ma, Abdul Ghaffar Memon, Hanchang Shi, Yi Qian. An array fluorescent biosensor based on planar waveguide for multi-analyte determination in water samples. Sensors and Actuators B: Chemical, 2017, 240, 107-113.